Get Help Sign In

Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

I am not getting any amplification. What might be causing this?

There could be many different explanations for not getting any amplification. A robust assay design is fundamental to qPCR success. Please see Design efficient PCR and qPCR primers and probes using online tools for more information about designing primers and probes for qPCR analysis.