Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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I currently use the KASP genotyping system. Can I directly substitute PACE Genotyping Master Mix and IDT primers?

Yes! You can use the PACE™ Genotyping Master Mix (3CR Bioscience) and IDT primers with the same cycling conditions used with KASP™ assays (LGC Biosearch Technologies).

You would likely, however, obtain better quality results by using an alternative touchdown phase of 10 cycles of denaturation at 94°C for 20 seconds, and annealing/extension at 65°C–57°C for 60 seconds, dropping 0.8°C per cycle.


Categories:
qPCR & PCR