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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

I currently use the KASP genotyping system. Can I directly substitute PACE Genotyping Master Mix and IDT primers?

Yes! You can use the PACE™ Genotyping Master Mix (3CR Bioscience) and IDT primers with the same cycling conditions used with KASP™ assays (LGC Biosearch Technologies).

You would likely, however, obtain better quality results by using an alternative touchdown phase of 10 cycles of denaturation at 94°C for 20 seconds, and annealing/extension at 65°C–57°C for 60 seconds, dropping 0.8°C per cycle.


Categories:
qPCR & PCR

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.