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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

In what direction does IDT synthesize oligos?

We synthesize our oligos in the 3' -> 5' direction off of a controlled-pore glass (CPG) solid support using amidite chemistry.

This 3' -> 5' technique mitigates the effect of truncated product on primer binding, as it will be limited to the 5' end.

Custom DNA & RNA