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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Is library amplification required for xGen™ Broad-Range RNA Library Prep Kit? How many cycles of PCR is needed with the xGen Broad-Range Library Prep Kit?
Yes.
The Indexing PCR step is a required step in order to complete the indexed adapter sequences and generate sufficient library for sequencing.
Please refer to the xGen Broad-Range RNA Library Prep Kit Protocol protocol for specific cycle requirements based on input RNA quantity.
Categories:
Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.