Processing
Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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Is library amplification required for xGen™ Broad-Range RNA Library Prep Kit? How many cycles of PCR is needed with the xGen Broad-Range Library Prep Kit?
Yes.
The Indexing PCR step is a required step in order to complete the indexed adapter sequences and generate sufficient library for sequencing.
Please refer to the xGen Broad-Range RNA Library Prep Kit Protocol protocol for specific cycle requirements based on input RNA quantity.