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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Is the xGen™ Methyl-Seq DNA Library Prep Kit compatible with reduced representation bisulfite sequencing (RRBS)?
Yes.
The chemistry of xGen Methylation Sequencing easily works in an RRBS workflow. To capture the fraction of DNA fragments that are enriched for CpG islands, this RRBS protocol requires that size selection of inserts occurs after MspI DNA digestion, but before bisulfite conversion.
As the xGen Methyl-Seq DNA Library Prep Kit requires bisulfite conversion prior to library preparation, size selection must be performed on the DNA fragments immediately following MspI digestion (rather than on library molecules, as in the traditional workflow).
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Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.