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The chemistry of xGen Methylation Sequencing easily works in an RRBS workflow. To capture the fraction of DNA fragments that are enriched for CpG islands, this RRBS protocol requires that size selection of inserts occurs after MspI DNA digestion, but before bisulfite conversion.
As the xGen Methyl-Seq DNA Library Prep Kit requires bisulfite conversion prior to library preparation, size selection must be performed on the DNA fragments immediately following MspI digestion (rather than on library molecules, as in the traditional workflow).
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