Is there a minimum or maximum limit to the number of samples that can be placed into a single Normalase™ pool?
No, there is not a minimum or maximum limit to the number of sample libraries that can be combined into a single Normalase™ pool. However, consider your desired number of reads for each sample and only pool together those samples that have the same required read counts.
Additionally, consider index compatibility as well as insert size. Pool libraries of comparable insert sizes that can be co-sequenced to avoid size dependent clustering effects that are independent of molarity and can lead to higher variation of sample representation in sequencing data.
Note: Do not pool libraries with index combinations that have not been validated by the supplier or risk demultiplexing errors and loss of data.