Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Is there a modification you would recommend to block the 3' end of an oligo?
There are several modifications that can block 3' extension. Most 3' modifications will block extension during PCR. The modifications most frequently used for this purpose are the 3'ddC, 3' Inverted dT, 3' C3 spacer, 3' Amino, and 3' phosphorylation.