Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

FAQ categories

CRISPR genome editing 
Custom DNA & RNA 
Functional genomics 
Genes & gene fragments 
Next generation sequencing 
Optional services 
qPCR & PCR 
Reagents & kits 

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Is titration of adapters required for different input amounts when using the Lotus DNA Library Prep Kit?

The adapter concentration used in library preparation varies depending on the amount of input dsDNA. For high-quality input gDNA, the recommended adapter concentrations are outlined in the following table:

DNA input (ng) IDT Adapter dilution Stock concentration (µM) 
≥25 No dilution 15 
10 10-fold (1:10) 1.5 
1 20-fold (1:20) 0.75 

Categories:
Next generation sequencing

Tags:
ngs hybrid capture target capture