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The adapter concentration used in library preparation varies depending on the amount of input dsDNA. For high-quality input gDNA, the recommended adapter concentrations are outlined in the following table:
DNA input (ng) | IDT Adapter dilution | Stock concentration (µM) |
≥25 | No dilution | 15 |
10 | 10-fold (1:10) | 1.5 |
1 | 20-fold (1:20) | 0.75 |