Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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Is titration of adapters required for different input amounts when using the Lotus DNA Library Prep Kit?

The adapter concentration used in library preparation varies depending on the amount of input dsDNA. For high-quality input gDNA, the recommended adapter concentrations are outlined in the following table:

DNA input (ng) IDT Adapter dilution Stock concentration (µM) 
≥25 No dilution 15 
10 10-fold (1:10) 1.5 
1 20-fold (1:20) 0.75