Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
The adapter concentration used in library preparation varies depending on the amount of input dsDNA. For high-quality input gDNA, the recommended adapter concentrations are outlined in the following table:
DNA input (ng) | IDT Adapter dilution | Stock concentration (µM) |
≥25 | No dilution | 15 |
10 | 10-fold (1:10) | 1.5 |
1 | 20-fold (1:20) | 0.75 |
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.