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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Is titration of adapters required for different input amounts with the xGen™ DNA Library Prep Kit EZ / MC?
For direct sequencing of libraries prepared with the xGen™ DNA Library Prep Kit EZ from DNA inputs less than 25 ng, adapter dilution is necessary to obtain low levels of adapter dimers.
Therefore, careful quantification of your sample input is necessary for attaining ideal results. Recommended adapter titration is included in the xGen DNA Library Prep EZ protocol ligation instructions.
For direct sequencing of libraries prepared with the xGen DNA Library Prep Kit MC from DNA inputs less than 50 ng, adapter dilution is necessary to attain low levels of adapter dimers. Adapter titration recommendations are included in the library prep protocol ligation section. For intermediate inputs, scale the adapter dilution accordingly. Adjustments may be required.
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