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For direct sequencing of libraries prepared with the xGen™ DNA Library Prep Kit EZ from DNA inputs less than 25 ng, adapter dilution is necessary to obtain low levels of adapter dimers.
Therefore, careful quantification of your sample input is necessary for attaining ideal results. Recommended adapter titration is included in the xGen DNA Library Prep EZ protocol ligation instructions.
For direct sequencing of libraries prepared with the xGen DNA Library Prep Kit MC from DNA inputs less than 50 ng, adapter dilution is necessary to attain low levels of adapter dimers. Adapter titration recommendations are included in the library prep protocol ligation section. For intermediate inputs, scale the adapter dilution accordingly. Adjustments may be required.
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