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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Should I amplify my eBlocks™ Gene Fragments when I receive them?
We do not recommend amplifying after receipt. eBlocks Gene Fragments are synthesized from several smaller overlapping fragments. Even after purification, the final prep may contain some of these smaller byproducts.
During PCR smaller products are more efficiently amplified than longer ones, and once the exponential phase of PCR is reached, any smaller products in the reaction will overwhelm the generation of the full-length correct product. Researchers will often see a smear or smaller bands than expected when the PCR product is run on a gel.
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Genes & gene fragments*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.