Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Should I only be concerned about sodium ions when calculating Tm?
Any charge will play a role in the Tm value for an oligonucleotide in a reaction. Thus, sodium, magnesium, and potassium concentrations each contribute to this calculation.
The IDT OligoAnalyzer® tool will calculate Tm for you, taking into account the presence of specific ions in the reaction buffer. Find this program under the "SciTools®" tab of the IDT website.