Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Search all FAQs:

Should I use the Alt-R® Cas9 D10A or H840A nickase?

It depends upon the experimental design. Cas9 D10A will cut the target strand of DNA (which does not contain the PAM sequence), while Cas9 H840A will cut the non-target strand (which contains the PAM sequence). The availability and location of PAM sites within the target region of DNA needs to be assessed. To learn more about the design and use of Cas9 nickases, refer to the application note at www.idtdna.com/CRISPR-Cas9 (Resources section).