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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

There are no PAM NGG sequences close to my locus of interest. What can I do?

If the region of interest is so narrow that there are no “GG” motifs, you may have to explore Cas9 endonucleases (from other organisms) that recognize different PAM sites. [Jiang et al. (2013) RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotech, 31:233–239.] has also shown that S. pyogenes Cas9 recognizes NAG, though with less efficiency. Note that our research group is not focused on testing these approaches. However, your are welcome to contact us to discuss what we have learned based on ongoing research. Learn more about our Alt-R CRISPR-Cas9 System products.