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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Which adapters can I use with this kit?
This kit includes our stubby Y Adapters that can be utilized together with a variety of different indexing primer kits supplied in tube or plate formats by IDT. These include unique dual indexing solutions from 96-plex to 1536-plex, and if using the Normalase™ workflow, be sure to select Normalase indexing primers.
For indexing by ligation, which is compatible with PCR-free sequencing, we recommend either the xGen™ DNA Library Prep Kit EZ UNI or the xGen DNA Library Prep Kit MC UNI together with Illumina® TruSeq® DNA Single Indexes, TruSeq® DNA CD (combinatorial dual), or IDT for Illumina® UD (unique dual) DNA Indexes. PCR primers are included in the kit for the optional PCR workflow. If using the Normalase workflow, be sure to use the Normalase amplification primers in the Normalase kit.
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Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.