Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What applications can xGen UDI-UMI Adapters be used for?

The xGen UDI-UMI Adapters can be used in applications which utilize TA-ligation workflows such as:

  • Whole genome sequencing (WGS)
  • PCR-free sequencing
  • Detection of germline, inherited single nucleotide polymorphisms (SNPs) and insertions/deletions (indels)
  • Hybridization capture of target sequences (e.g., the exome or other regions of interest)
  • Low-frequency somatic variant detection of SNPs and indels
  • RNA-seq
  • Metagenomic sequencing
  • Methyl-Seq*

    * Because the xGen Methyl UDI-UMI Adapters are methylated, bisulfite conversion can be carried out after the ligation (of the adapter), but before library amplification.

Due to the UMI present on the adapter, the xGen UDI-UMI Adapters are especially suited for low-frequency variant detection (down to <1%), as well as counting applications (e.g., RNA-seq).

Note: Strand-specific libraries constructed using the xGen UDI-UMI Adapters have the insert strandedness flipped (i.e., to the opposite strand) when compared to standard TruSeq™-Compatible Full-length Adapters. This strand flip must be accounted for in data analysis for strand-specific applications (e.g., stranded RNA-seq, bisulfite-seq).

TruSeq is a trademark of Illumina, Inc. and is used with permission.



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