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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What are the main steps for library construction using the xGen™ Broad-Range Library Prep Kit?

The xGen™ Broad-Range RNA Library Prep Kit uses 5 main steps:

  1. Fragmentation & Reverse Transcription, where RNA is fragmented and converted to 1st strand cDNA
  2. Adaptase, where the Read 2 Stubby Adapter is added to the 3’ end of 1st strand cDNA
  3. Extension, where a primer extension reaction generates a dsDNA duplex
  4. Ligation, where the Read 1 Stubby Adapter is ligated to the duplex
  5. Indexing PCR, where the fully indexed adapter sequences are added, and the library is amplified to generate sufficient yield for sequencing.


*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.