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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What are the main steps for library construction using the xGen™ RNA Library Prep Kit?

The xGen™ RNA Library Prep Kit uses three main steps:

  1. Fragmentation & Reverse Transcription, where RNA is fragmented and converted to cDNA. Because the random primer is tailed, the 1st strand cDNA already contains the Read 1 Stubby Adapter.
  2. Adaptase, where the Read 2 Stubby Adapter is added to the 3’ end of 1st strand cDNA.
  3. Indexing PCR, where simultaneously the fully indexed adapter sequences are added and the library is amplified to generate sufficient yield for sequencing.


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