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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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What are the major steps in the xGen™ DNA Library Prep Kit EZ library construction?
Enzymatic prep. Consists of fragmentation of dsDNA followed by end-repair and dA-tailing of the fragments—all performed in a single reaction.
Adapter Ligation. Stubby or full-length Y adapters are ligated to 3’-dA-tailed molecules.
Library amplification. Employs a high-fidelity, low-bias polymerase. For indexing using the ligation workflow, amplification primers can be used, but PCR is optional when using the minimum of 100 ng input. For indexing by PCR, stubby Y adapters are used during ligation and amplified with indexing primers to complete the adapter sequence and add sample indexes.
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Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.