Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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What causes over-fragmented DNA during sequencing library construction?

High temperatures

Over-fragmentation can occur if the reaction is left at room temperature instead of being kept on ice.

Compromised sample integrity

Samples with compromised integrity are also more prone to over-fragmentation.