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The Alt-R HDR Enhancer Protein has been shown to increase HDR rates in a variety of cell types (U2OS cells, Jurkat cells, HEK 293 cells, iPSCs, HSPCs, and T cells) and using a variety of donor types including ssDNA, linear dsDNA, plasmid DNA, and AAV template. The level of increase is often dependent on the combination of the cell line and donor type. For example, while the Alt-R HDR Enhancer Protein has been shown to boost HDR rates in T cells with non-viral donors and in HSPCs with an AAV donor, it has low effectiveness when used in T cells with an AAV donor. We recommend testing a range of doses with your cell line and donor to determine the level of effectiveness of the Alt-R HDR Enhancer Protein in your specific application.