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What conditions should I use to HPLC purify my oligos?
IDT uses our own proprietary HPLC method to purify oligos in-house. A published set of conditions for a RP-HPLC protocol is: Hamilton PRP-1 Reverse Phase column 250x10 mm, Buffer A = 100% ACN (acetonitrile), Buffer B = ACN/0.1M TEAA pH 7.3. Run at 4 mil/min over 30 mim from 95–60% A. Monitor at 260/297 nm.