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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What conditions should I use to HPLC purify my oligos?

IDT uses our own proprietary HPLC method to purify oligos in-house. A published set of conditions for a RP-HPLC protocol is:
- Hamilton PRP-1 Reverse Phase column 250x10 mm
- Buffer A = 100% ACN (acetonitrile)
- Buffer B = ACN/0.1M TEAA pH 7.3.
- Run at 4 mil/min over 30 mim from 95–60% A. Monitor at 260/297 nm.

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