Get Help Sign In

Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What conditions should I use to HPLC purify my oligos?

IDT uses our own proprietary HPLC method to purify oligos in-house. A published set of conditions for a RP-HPLC protocol is:
- Hamilton PRP-1 Reverse Phase column 250x10 mm
- Buffer A = 100% ACN (acetonitrile)
- Buffer B = ACN/0.1M TEAA pH 7.3.
- Run at 4 mil/min over 30 mim from 95–60% A. Monitor at 260/297 nm.

Custom DNA & RNA

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.