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What cycling parameters will give me the best results when using the PrimeTime® Gene Expression Master Mix?
The master mix can be used with either standard or fast cycling conditions, depending on what qPCR system you are using. For best results, use one of the cycling parameters provided in the protocol,
PrimeTime Gene Expression Master Mix:
Standard cycling conditions: 3 min. 95°C; 35–45 x (15 sec. 95°C; 1 min. 60°).
Fast cycling conditions: 3 min. 95°C; 35–45 x (5 sec. 95°C; 30 sec. 60°).