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If variability is observed, consider the following factors:
1. Mixing and handling: ensure thorough and consistent mixing of capture reactions to maintain homogeneity throughout the workflow. Thorough mixing is vital for consistent results.
2. Input consistency: verify that library input amounts are within the recommended range (100 – 6000ng total) and are evenly distributed across replicates
3. Library quality: differences in DNA quality may contribute to variability, although this workflow has been shown to perform robustly across challenging sample types
4. Sequencing depth: ensure sufficient and consistent sequencing depth across samples, particularly when multiplexing
5. General workflow consistency: maintain consistent timing, mixing, preparations steps