What guarantees are associated with the TriFECTa kit? Is the Predesigned DsiRNA specific?
IDT guarantees that at least 2 of the 3 DsiRNAs in the TriFECTa Kit
will result in at least 70% knockdown of the target mRNA when the following conditions are met: 1) the DsiRNAs are used at 10 nM concentration, 2) results are assayed by quantitative RT-PCR, 3) the fluorescent transfection control DsiRNA indicates that >90% of the cells have been transfected, and 4) the HPRT positive control DsiRNA works with the expected efficiency.
Our Bioinformatics Department does a full screening of sequence designs before adding them to the database. This includes performing a BLAST search of the sequences to ensure they will only target the gene in question, performing secondary structure checks, and making sure the end stabilities are favorable for incorporation into the RISC complex.