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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What indexing primers can I use with this xGen™ RNA Library Prep Kit?

The xGen™ RNA Library Prep Kits have an Indexing PCR step to complete the fully indexed adapter sequences following ligation of stubby adapters during the library prep workflows so they are fully compatible with Illumina sequencers.

IDT supplies a variety of index configurations and strategies, including dual indexing as either:

  • Combinatorial dual indexing, up to 96 combinations
  • Unique dual indexing, up to 1536 unique dual indices


*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.