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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Which insert sizes are appropriate to use with the Normalase™ protocol?

The best results are obtained by using libraries with similar median insert sizes (i.e., 200−350 bp inserts), as measured by fragment analysis.

Libraries with broad or variable size distribution (i.e., transposase-based workflows) that demonstrate size-dependent clustering effects on Illumina sequencers and are independent of molarity, may have more variable results.

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