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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What instruments are required to run the Sherlock™ CRISPR SARS-CoV-2 kit?

The Sherlock™ CRISPR SARS-CoV-2 kit requires a heat block capable of maintaining 61⁰C and a BioTek model NEO2 microplate reader running Gen5 3.08 software, capable of fluorescence detection at 37°C (ex/em 490 nm/520 nm).

Additionally, standard equipment found in molecular laboratories (vortexer, minicentrifuge, pipettes, Biosafety Cabinet Class II for the extraction, PCR workstations for each portion of the assay setup, and a heat block with a heated lid capable of maintaining 61°C or a PCR instrument with a heated lid) are required.