CRISPRAltRations is the analysis platform behind the rhAmpSeq CRISPR Analysis Tool. This IDT-developed analysis pipeline is used for CRISPR on- and off-target editing analysis of next generation sequencing (NGS) data generated from amplicon resequencing. The analysis platform¾available through the rhAmpSeq CRISPR Analysis Tool utilizes cloud-hosted computational resources for data processing. Briefly, this is the workflow:
Although this workflow is relatively common to most software tools that analyze NGS data derived from CRISPR screens, the rhAmpSeq CRISPR Analysis Tool has key improvements that enable higher accuracy of variant detection, including:
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