Get Help Sign In
ProcessingProcessing

Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What is the average frequency of the CRISPR-Cas9 PAM sequence in the mammalian genome?

Using the frequency of “GG” = 5.21% in the reference human genome [1], there is an expected 161,284,793 NGG PAM sites in the human genome, or approximately one “GG” dinucleotide every 42 bases.

Reference

  1. Scherer S (2008) A short guide to the human genome. Cold Spring Harbor, NY:Cold Spring Harbor Laboratory Press. xiv, p173.


*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.