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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What is the library yield requirement for expected functionality with the Normalase™ Module?

Obtaining a minimum threshold of 6 nM or 12 nM in a 20 µL volume after PCR, depending on the chosen workflow option, is required for expected functionality. Libraries that do not meet this threshold will be less than 2 nM or 4 nM post-Normalase Module (from 6 nM or 12 nM minimum thresholds, respectively) and will be under-represented during cluster generation.

Additionally, if multiple libraries are under 12 nM to start, the final library pool will be < 4 nM, resulting in potential under clustering of the pool on the flow cell. This is similarly the case for the 6 nM normalization workflow, where the final library pool will be < 2 nM resulting in under clustering of the pool on the flow cell.



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