Get Help Sign In

Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What is the library yield requirement for expected functionality with the Normalase™ Module?

Obtaining a minimum threshold of 6 nM or 12 nM in a 20 µL volume after PCR, depending on the chosen workflow option, is required for expected functionality. Libraries that do not meet this threshold will be less than 2 nM or 4 nM post-Normalase Module (from 6 nM or 12 nM minimum thresholds, respectively) and will be under-represented during cluster generation.

Additionally, if multiple libraries are under 12 nM to start, the final library pool will be < 4 nM, resulting in potential under clustering of the pool on the flow cell. This is similarly the case for the 6 nM normalization workflow, where the final library pool will be < 2 nM resulting in under clustering of the pool on the flow cell.

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.