Processing
Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Search all FAQs:
What is the optimal length of a primer?
IDT recommends you aim for primers between 18–30 bases; however, the most important considerations for primer design should be their Tm value and sequence specificity. Primers should also be free of strong secondary structures and self-complementarity.
The free IDT OligoAnalyzer™ Tool can be used to evaluate these features.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.