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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What is the standard desalting procedure IDT uses for newly synthesized oligos, and what additional purification options do I have?

IDT uses a proprietary desalting technique that removes some truncation products and small organic contaminants from the synthesized oligonucleotide preparation. Removal of n-1mers produced during synthesis requires additional PAGE or HPLC purification. PAGE is recommended for unmodified oligos >80-100 bases, while HPLC is the preferred method for oligos modified with either fluorescent dyes or attachment chemistries.