If the product will be used in a ligation reaction, the addition of 5’-phosphate may be needed. This can be done at the time of oligo synthesis (chemical phosphorylation) or at any time thereafter using PNK (enzymatic phosphorylation). If the oligos are relatively long or will be used in cloning, starting with PAGE-purified oligos is recommended.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.