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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What limit of detection (LOD) is achievable with for xGen™ HS EGFR Pathway Amplicon Panel?

xGen HS EGFR Pathway Amplicon Panel help to enable variant calling at and below 1% frequency, with allele frequency dependent on the amount of input material available and the depth of sequencing.

Using 10 ng of input DNA, the xGen HS EGFR Pathway Amplicon Panel consistently identifies variants at 0.5% with a recommended sequencing depth of at least 60,000x (1M reads) prior to deduplication with UMIs and 0.25% from 20 ng of input DNA. Read more here.



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