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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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What limit of detection (LOD) is achievable with the xGen™ HS EGFR Pathway Amplicon Panel?
The xGen HS EGFR Pathway Amplicon Panel helps to enable variant calling at and below 1% frequency, with allele frequency dependent on the amount of input material available, and the depth of sequencing.
Using 10 ng of input DNA, the xGen HS EGFR Pathway Amplicon Panel consistently identifies variants at 0.5%, with a recommended sequencing depth of at least 60,000x (1M reads), before deduplication with UMIs and 0.25% from 20 ng of input DNA.
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Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.