Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

FAQ categories

CRISPR genome editing 
Custom DNA & RNA 
Functional genomics 
Genes & gene fragments 
Next generation sequencing 
Optional services 
qPCR & PCR 
Reagents & kits 

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What master mix does IDT recommend for use with PrimeTime® qPCR Assays?

IDT recommends the Brilliant III qPCR Master Mix from Agilent. IDT has also tested other master mixes and found that PrimeTime qPCR Assays demonstrated efficiency close to 100% across many commercially available master mixes.

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