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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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What methods are supported for DNA fragmentation prior to library construction?
The xGen™ DNA Library Prep Kit MC was tested using Covaris sheared DNA but is compatible with other mechanical and enzymatic fragmentation methods that are commonly used in NGS library preparation workflows.
If you prefer not to use mechanical shearing, or do not have access to specialized equipment, we recommend using the xGen™ DNA Library Prep Kit EZ with enzymatic fragmentation.