Your product is now available from Integrated DNA Technologies.
Many of the Swift products you have grown to love are now part of our new complete portfolio, xGen™ NGS. Through this new partnership we are pleased to offer you comprehensive next generation sequencing solutions.
xGen NGS—made for you.
Unsure of what products are available? Or, perhaps you’d like guidance on which products are compatible? If so, try our xGen NGS Solutions Builder Tool today.
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
What reagents will I need in addition to the Alt-R™ RNAs to do a CRISPR genome editing experiment?
The Alt-R™ CRISPR-Cas9 System provides custom CRISPR crRNA and a universal CRISPR tracrRNA, both of which are required for CRISPR-Cas9 genome editing using the Alt-R CRISPR-Cas9 System protocol.
A Nuclease-Free Duplexing Buffer used to complex the crRNA and the tracrRNA is included with the tracrRNA. You will also need S. pyogenes Cas9 endonuclease. Alt-R S.p. Cas9 Expression Plasmid can be transfected into cells for expression of this endonuclease. Supply your own transfection reagents that will effectively deliver RNA and DNA into your cell lines.
We also highly recommend the use of controls, which can be ordered as part of the Alt-R™ CRISPR-Cas9 System.