Processing
Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
What reverse transcription (RT) module is recommended?
The xGen™ SARS-CoV-2 Amplicon Panel used with the xGen Amplicon Core Kit and indexing primers supports first- or second-strand cDNA, generated from the SARS-CoV-2 RNA genome, as input.
Select a cDNA synthesis module that supports two-step RT-PCR, includes random primers, and has a processivity of >1 kb.
The panel has been tested with Superscript® IV First-Strand Synthesis System (Thermo Fisher Cat. No. 18091050). The manufacturer’s protocol was followed as written using random primers and associated specifications. Steps involving the optional host gDNA/RNA removal and RNAse H were not performed.
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Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.