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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What should I do if my HDR edit did not work?

Editing rates and HDR efficiency can vary significantly from system to system and target to target. Strategies for increased HDR efficiency can include using a guide RNA with robust editing activity, adding the Alt-R™ HDR Enhancer V2, and optimizing donor template design. Some troubleshooting measures are to include a positive control, increase the number of clonal isolates screened, and ensure that the intended edit is not lethal, or being selected against. Contact us at applicationsupport@idtdna.com for further assistance.



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