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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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What should I do if the IDT online ordering tool says that my sequence is too complex to order, but I need it as is?
In most cases, our system has found a region with either very high G/C content or several small repeats that would prevent us from synthesizing your sequence.
If your sequence contains a coding region, you can use codon optimization to reduce the complexity. Codon optimization uses synonymous codons to retain the final amino acid sequence that is expressed while generating a sequence that we can synthesize.
If your sequence is not a coding region, we might be able to synthesize the sequence as a custom gene, which is delivered in a basic pUC vector, rather than just a linear, dsDNA fragment. For assistance, send your sequences and any questions to genes@idtdna.com.
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Genes & gene fragments*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.