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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What type of purification do I need to select for my 454 sequencing primer synthesis?

Roche recommends IDT HPLC-purified 454 Fusion Primers to ensure that you get the best data from your sequencing runs.

The high purity level of HPLC-purified primers minimizes the risk of mispriming events due to truncations and other errors that may compromise sequencing data quality or the number of viable reads that can be obtained from a single Pico Titer Plate run.

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*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.