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For best results, use the cycling parameters provided in the PrimeTime One-Step 4X Broad-Range Master Mix Protocol.Fast cycling conditions: 15 min. 50°C; 30 sec. 95°C; 35–45x (5 sec. 95°C, 45 sec. 60°C).
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.