Your product is now available from Integrated DNA Technologies.
Many of the Swift products you have grown to love are now part of our new complete portfolio, xGen™ NGS. Through this new partnership we are pleased to offer you comprehensive next generation sequencing solutions.
xGen NGS—made for you.
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Welcome to the IDT family!
Find Archer now at IDT!
All Archer information is now available on IDT’s website. You can view Archer assays alongside IDT’s xGen™ NGS portfolio to find the best next generation sequencing solution for your lab.
Confidently detect more with Archer NGS assay solutions for your solid tumor, blood cancer, immune profiling, and genetic disease research.
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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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NHEJ vs HDR: Which repair pathway to repair CRISPR mediated double stranded breaks?
In most eukaryotic cells, the non-homologous end joining (NHEJ) pathway generates insertions and deletions during double-stranded break (DSB) repair. However, in the presence of a DNA template with homology to the sequences flanking the DSB location, homology-directed repair (HDR) can seal the DSB in an error-free manner .
Is NHEJ or HDR better?
In most cells, both repair pathways are active, however the HDR pathway is generally less efficient than the NHEJ pathway in the absence of a homologous template. This template is naturally present as sister chromatin in late S phase and G2 phase , and may be added artificially as donor DNA [3,4].
The efficiency of HDR is determined by the concentration of donor DNA present at the time of repair, the length of the homology arms of the donor DNA, the cell cycle, and the activity of the endogenous repair systems . These factors contribute to the high variability of HDR efficiency observed across cell lines, particularly in immortalized cells . It is important to determine the optimal HDR conditions for each cell line.