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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Why do the scale I order and the amount of oligo I receive differ—I receive less than the requested scale quantity?

The scale you order determines the amount of sequence-initiating base used to begin synthesis of your oligonucleotide. Since oligonucleotide synthesis (or any chemical reaction) is never 100% efficient, the amount of final product recovered after synthesis and purification steps (yield) will always be less than the starting amount used for synthesis. Modifications, secondary structures, and purification services further affect the final yield for a given construct.

IDT offers guaranteed yields based on all of these factors that define the minimum amount of oligonucleotide you will receive for a given construct.
The minimum yield guarantee is listed in the shopping cart and in your confirmation email.

To receive a higher final yield, select a larger synthesis scale. For further discussion on this topic, see the Technical Report, Oligonculdotide Yield, Resuspension, and Storage.


*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.