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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Why does the xGen™ cfDNA & FFPE Library Prep Kit have fixed instead of random UMIs?

Fixed UMI sequences help to enable identification and correction of sequencing or PCR errors, even if they appear within the UMI sequence.

During error correction, using fixed UMI sequences reduces the chance that an error in UMI sequences will accidently be counted as a separate original molecule.

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.