Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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Why does the xGen Prism DNA Library Prep Kit have fixed instead of random UMIs?

Fixed UMI sequences enable identification and correction of sequencing or PCR errors, even if they appear within the UMI sequence. During error correction, using fixed UMI sequences greatly reduces the chance that an error in UMI sequences will accidently be counted as a separate original molecule.


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