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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Why is TruGrade processing useful in my NGS experiments?

TruGrade® Processing reduces the risk of oligonucleotide crosstalk during multiplex next generation sequencing (NGS) applica­tions. Oligo crosstalk is a potential cause of barcode misalignment, which can lead to inaccurate conclusions drawn from associat­ing sequencing data with the wrong sample.

We recommend TruGrade Processing for sample preparation using barcoded adapt­ers for multiplex sequencing, and for PCR using barcoded fusion primers for multiplex amplicon sequencing.


*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.