Frequently asked questions

TruGrade^® Processing reduces the risk of oligonucleotide crosstalk during multiplex next generation sequencing (NGS) applica­tions. Oligo crosstalk is a potential cause of barcode misalignment, which can lead to inaccurate conclusions drawn from associat­ing sequencing data with the wrong sample. We recommend TruGrade Processing for sample preparation using barcoded adapt­ers for multiplex sequencing, and for PCR using barcoded fusion primers for multiplex amplicon sequencing.