Processing
Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Why is TruGrade processing useful in my NGS experiments?
TruGrade® Processing reduces the risk of oligonucleotide crosstalk during multiplex next generation sequencing (NGS) applications. Oligo crosstalk is a potential cause of barcode misalignment, which can lead to inaccurate conclusions drawn from associating sequencing data with the wrong sample. We recommend TruGrade Processing for sample preparation using barcoded adapters for multiplex sequencing, and for PCR using barcoded fusion primers for multiplex amplicon sequencing.