Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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Why should I sequence multiple colonies when cloning?

Sometimes researchers will pick just a colony or two for sequencing. If they find problems with their clone, they may conclude something must be wrong with their primers when they simply have picked a bad colony. IDT recommends sequencing at least 8–10 separate colonies to get a realistic representation of the clonal population and to provide a good chance of finding the correct sequence if one exists.