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Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Will polyacrylamide gel electrophoresis damage a Cy-labeled oligonucleotide?
We do not recommend PAGE purification of Cy-labeled oligonucleotides. Ammonium persulfate, a cross linking agent used in place of urea, is a component of the acrylamide gel that can damage the dye. Ammonium persulfate oxidizes the double bonds in the chain between the rings, affecting the dye's ability to flouresce when excited. HPLC purification, however, does not oxidize Cy dyes and is required for these modifications.