Order Menu

User guides and protocols

DNA & RNA

PDF tag

Reduction of thiol-modified oligonucleotides (575 KB)

PDF tag

Annealing oligonucleotides (503 KB)

PDF tag

Resuspension of duplexed oligonucleotides (267 KB)

CRISPR genome editing

PDF tag

Alt-R CRISPR-Cas9 system—in vitro cleavage of target DNA with RNP complex (358 KB)

PDF tag

Alt-R CRISPR-Cas9 system—RNP electroporation, Gene Pulser Xcell system (436 KB)

PDF tag

Alt-R CRISPR-Cas9 system—RNP transfections (recommended) (696 KB)

PDF tag

Alt-R CRISPR-Cas9 system—plasmid transfections (844 KB)

PDF tag

Alt-R CRISPR-Cpf1—RNP electroporation, Amaxa Nucleofector system (768 KB)

PDF tag

Alt-R CRISPR-Cpf1—RNP electroporation, Neon Transfection system (319 KB)

PDF tag

Alt-R CRISPR-Cas9 system—RNP electroporation, Amaxa Nucleofector system (491 KB)

PDF tag

Alt-R CRISPR-Cas9 system—RNP electroporation, Neon Transfection system (376 KB)

PDF tag

Alt-R Genome Editing Detection Kit (910 KB)

User-submitted methods

PDF tag

CRISPR-Cas9 RNP delivery—mouse zygote electroporation—NepaGene (158 KB)

PDF tag

CRISPR-Cas9 RNP delivery—iPS cell electroporation—NepaGene (210 KB)

PDF tag

CRISPR-Cas9 RNP delivery—human stem cells—K Moore (165 KB)

PDF tag

CRISPR-Cas9 RNP delivery—mouse zygote microinjection—CB Gurumurthy (505 KB)

PDF tag

CRISPR-Cas9 RNP delivery—zebrafish embryos—J Essner (119 KB)

PDF tag

CRISPR-Cas9 RNP delivery—mouse zygote electroporation—Takemoto (176 KB)

PDF tag

CRISPR-Cas9 RNP delivery—C. elegans—A Dernburg (123 KB)

Next generation sequencing

xGen hybridization capture of DNA libraries for NGS target enrichment (3926 KB)

Automation of xGen hybridization capture on the Sciclone G3 NGS Workstation (4302 KB)

Legacy protocols

PDF tag

Hybridization capture of Nextera DNA libraries using xGen Lockdown Probes and Panels (490 KB)

PDF tag

xGen Blocking Oligos resuspension protocol (506 KB)

PDF tag

xGen Lockdown Probes and Panels resuspension protocol (664 KB)

PDF tag

Expansion of xGen Lockdown Panels with xGen spike-in panels (681 KB)

PDF tag

Hybridization capture of TruSeq libraries using xGen Lockdown Probes and Reagents (530 KB)

Data analysis guidelines

PDF tag

Demultiplexing data containing unique molecular indexes (UMIs) (728 KB)

PDF tag

Variant calling data containing unique molecular indexes (UMIs) (1813 KB)

Genes & gene fragments

PDF tag

gBlocks Gene Fragments (2376 KB)

PDF tag

gBlocks Gene Fragment T/A cloning (141 KB)

PDF tag

Gibson Assembly method (178 KB)

qPCR & PCR

PDF tag

rhAmp SNP Genotyping (670 KB)

PDF tag

rhPCR (436 KB)

PDF tag

Dye calibration—AB 7300 and 7500/7500 Fast (139 KB)

PDF tag

PrimeTime qPCR Assay Plate resuspension (507 KB)

PDF tag

PrimeTime Gene Expression Master Mix (149 KB)

PDF tag

Dye calibration—AB 7900 Fast (179 KB)

PDF tag

PrimeTime Primer Plate resuspension (500 KB)

PDF tag

PrimeTime qPCR Assay resuspension (504 KB)

PDF tag

PrimeTime qPCR Primers resuspension (499 KB)

Functional genomics

PDF tag

IDT miRNA Inhibitors resuspension (496 KB)

PDF tag

TriFECTa RNAi kits (1044 KB)

PDF tag

Resuspension of duplexed oligonucleotides (267 KB)

Reagents & kits

PDF tag

Surveyor Kit for WAVE systems (801 KB)

PDF tag

Surveyor Kit for gel electrophoresis (481 KB)

PDF tag

DNaseAlert nuclease detection (1399 KB)

PDF tag

RNaseAlert nuclease detection (609 KB)

PDF tag

Nuclease Decontamination Solution (495 KB)

PrimeTime Gene Expression Master Mix

PrimeTime-Gene-Expression-Master-Mix

Use promo code FREEMASTERMIX at checkout to receive a free vial of PrimeTime Gene Expression Master Mix (1 mL size).

lockup_1

lockup_2