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User guides and protocols

DNA & RNA

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Reduction of thiol-modified oligonucleotides (575 KB)

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Reduction of thiol-modified oligonucleotides (575 KB)

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Annealing oligonucleotides (503 KB)

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Annealing oligonucleotides (503 KB)

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Resuspension of duplexed oligonucleotides (267 KB)

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Resuspension of duplexed oligonucleotides (267 KB)

CRISPR genome editing

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Homology-directed repair using Alt-R CRISPR-Cas9 System and Ultramer Oligos (974 KB)

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Homology-directed repair using Alt-R CRISPR-Cas9 System and Ultramer Oligos (974 KB)

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Alt-R CRISPR-Cas9 system—RNP transfections (recommended) (1236 KB)

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Alt-R CRISPR-Cas9 system—RNP transfections (recommended) (1236 KB)

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Alt-R CRISPR-Cas9 system—RNP electroporation, Amaxa Nucleofector system (797 KB)

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Alt-R CRISPR-Cas9 system—RNP electroporation, Amaxa Nucleofector system (797 KB)

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Alt-R CRISPR-Cas9 system—RNP electroporation, Neon Transfection system (862 KB)

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Alt-R CRISPR-Cas9 system—RNP electroporation, Neon Transfection system (862 KB)

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Alt-R CRISPR-Cas9 system—RNP electroporation, Gene Pulser Xcell system (503 KB)

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Alt-R CRISPR-Cas9 system—RNP electroporation, Gene Pulser Xcell system (503 KB)

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Alt-R CRISPR-Cas9 system—in vitro cleavage of target DNA with RNP complex (528 KB)

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Alt-R CRISPR-Cas9 system—in vitro cleavage of target DNA with RNP complex (528 KB)

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Alt-R CRISPR-Cas9 system—plasmid transfections (430 KB)

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Alt-R CRISPR-Cas9 system—plasmid transfections (430 KB)

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Alt-R CRISPR-Cpf1—RNP electroporation, Amaxa Nucleofector system (514 KB)

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Alt-R CRISPR-Cpf1—RNP electroporation, Amaxa Nucleofector system (514 KB)

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Alt-R CRISPR-Cpf1—RNP electroporation, Neon Transfection system (496 KB)

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Alt-R CRISPR-Cpf1—RNP electroporation, Neon Transfection system (496 KB)

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Alt-R Genome Editing Detection Kit (910 KB)

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Alt-R Genome Editing Detection Kit (910 KB)

User-submitted methods

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CRISPR-Cas9 RNP delivery—P. falciparum—S Cobbold (333 KB)

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CRISPR-Cas9 RNP delivery—P. falciparum—S Cobbold (333 KB)

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CRISPR-Cas9 RNP delivery—human stem cells—K Moore (165 KB)

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CRISPR-Cas9 RNP delivery—human stem cells—K Moore (165 KB)

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CRISPR-Cas9 RNP delivery—iPS cell electroporation—NepaGene (210 KB)

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CRISPR-Cas9 RNP delivery—iPS cell electroporation—NepaGene (210 KB)

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CRISPR-Cas9 RNP delivery—CD34+ hematopoietic stem and progenitor cells—A Hendel (375 KB)

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CRISPR-Cas9 RNP delivery—CD34+ hematopoietic stem and progenitor cells—A Hendel (375 KB)

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CRISPR-Cas9 RNP delivery—mouse zygote electroporation—NepaGene (428 KB)

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CRISPR-Cas9 RNP delivery—mouse zygote electroporation—NepaGene (428 KB)

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CRISPR-Cas9 RNP delivery—mouse zygote electroporation—Takemoto (176 KB)

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CRISPR-Cas9 RNP delivery—mouse zygote electroporation—Takemoto (176 KB)

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CRISPR-Cas9 RNP delivery—mouse zygote microinjection—CB Gurumurthy (505 KB)

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CRISPR-Cas9 RNP delivery—mouse zygote microinjection—CB Gurumurthy (505 KB)

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CRISPR-Cas9 RNP delivery—zebrafish embryos—J Essner (119 KB)

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CRISPR-Cas9 RNP delivery—zebrafish embryos—J Essner (119 KB)

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CRISPR-Cas9 RNP delivery—B. tryoni microinjection—A Choo (179 KB)

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CRISPR-Cas9 RNP delivery—B. tryoni microinjection—A Choo (179 KB)

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CRISPR-Cas9 RNP delivery—C. elegans—A Dernburg (123 KB)

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CRISPR-Cas9 RNP delivery—C. elegans—A Dernburg (123 KB)

Next generation sequencing

Library prep

Lotus DNA Library Prep Kit Protocol (2441 KB)

Lotus DNA Library Prep Kit: Quick reference protocol - Targeted sequencing (1015 KB)

Lotus DNA Library Prep Kit: Quick reference protocol - PCR-amplified (1095 KB)

Lotus DNA Library Prep Kit: Quick reference protocol - PCR-free (914 KB)

Hybridization capture

xGen hybridization capture of DNA libraries for NGS target enrichment (3929 KB)

Automation of xGen hybridization capture on the Sciclone G3 NGS Workstation (2459 KB)

Legacy protocols—hybridization capture

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Hybridization capture of Nextera DNA libraries using xGen Lockdown Probes and Panels (490 KB)

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Hybridization capture of Nextera DNA libraries using xGen Lockdown Probes and Panels (490 KB)

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xGen Blocking Oligos resuspension protocol (506 KB)

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xGen Blocking Oligos resuspension protocol (506 KB)

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xGen Lockdown Probes and Panels resuspension protocol (664 KB)

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xGen Lockdown Probes and Panels resuspension protocol (664 KB)

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Expansion of xGen Lockdown Panels with xGen spike-in panels (681 KB)

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Expansion of xGen Lockdown Panels with xGen spike-in panels (681 KB)

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Hybridization capture of TruSeq libraries using xGen Lockdown Probes and Reagents (1960 KB)

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Hybridization capture of TruSeq libraries using xGen Lockdown Probes and Reagents (1960 KB)

Data analysis guidelines—hybridization capture

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Demultiplexing data containing unique molecular identifiers (UMIs) (1869 KB)

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Demultiplexing data containing unique molecular identifiers (UMIs) (1869 KB)

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Variant calling data containing unique molecular identifiers (UMIs) (1813 KB)

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Variant calling data containing unique molecular identifiers (UMIs) (1813 KB)

Amplicon sequencing

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High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (1235 KB)

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High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (1235 KB)

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Quick reference guide: High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (965 KB)

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Quick reference guide: High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (965 KB)

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rhAmpSeq library preparation for targeted amplicon sequencing (1620 KB)

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rhAmpSeq library preparation for targeted amplicon sequencing (1620 KB)

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Quick reference guide: rhAmpSeq library preparation for targeted amplicon sequencing (967 KB)

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Quick reference guide: rhAmpSeq library preparation for targeted amplicon sequencing (967 KB)

Data analysis guidelines—amplicon sequencing

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Analysis Guideline: Genotype and variant calling with rhAmpSeq sequencing data (1135 KB)

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Analysis Guideline: Genotype and variant calling with rhAmpSeq sequencing data (1135 KB)

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Analysis Guideline: Evaluate CRISPR DNA editing with rhAmpSeq sequencing data (903 KB)

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Analysis Guideline: Evaluate CRISPR DNA editing with rhAmpSeq sequencing data (903 KB)

Sample data—amplicon sequencing

Genotyping sample data—rhAmpSeq analysis (gz)

Download (41511 KB)

CRISPR sample data–rhAmpSeq analysis (gz)

Download (36133 KB)

Genes & gene fragments

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gBlocks Gene Fragment T/A cloning (141 KB)

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gBlocks Gene Fragment T/A cloning (141 KB)

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Gibson Assembly method (178 KB)

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Gibson Assembly method (178 KB)

qPCR & PCR

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rhAmp SNP Genotyping (670 KB)

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rhAmp SNP Genotyping (670 KB)

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rhPCR (436 KB)

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rhPCR (436 KB)

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Dye calibration—AB 7300 and 7500/7500 Fast (139 KB)

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Dye calibration—AB 7300 and 7500/7500 Fast (139 KB)

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PrimeTime qPCR Assay Plate resuspension (507 KB)

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PrimeTime qPCR Assay Plate resuspension (507 KB)

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PrimeTime Gene Expression Master Mix (149 KB)

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PrimeTime Gene Expression Master Mix (149 KB)

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Dye calibration—AB 7900 Fast (179 KB)

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Dye calibration—AB 7900 Fast (179 KB)

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PrimeTime Primer Plate resuspension (500 KB)

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PrimeTime Primer Plate resuspension (500 KB)

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PrimeTime qPCR Assay resuspension (504 KB)

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PrimeTime qPCR Assay resuspension (504 KB)

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PrimeTime qPCR Primers resuspension (493 KB)

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PrimeTime qPCR Primers resuspension (493 KB)

Functional genomics

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IDT miRNA Inhibitors resuspension (496 KB)

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IDT miRNA Inhibitors resuspension (496 KB)

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TriFECTa RNAi kits (1044 KB)

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TriFECTa RNAi kits (1044 KB)

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Resuspension of duplexed oligonucleotides (267 KB)

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Resuspension of duplexed oligonucleotides (267 KB)

Reagents & kits

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Surveyor Kit for gel electrophoresis (481 KB)

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Surveyor Kit for gel electrophoresis (481 KB)

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DNaseAlert nuclease detection (1399 KB)

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DNaseAlert nuclease detection (1399 KB)

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RNaseAlert nuclease detection (445 KB)

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RNaseAlert nuclease detection (445 KB)

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Nuclease Decontamination Solution (495 KB)

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Nuclease Decontamination Solution (495 KB)

PrimeTime Gene Expression Master Mix

qPCR gene expression master mix

Use promo code FREEMASTERMIX* at checkout to receive a free vial of PrimeTime Gene Expression Master Mix (1 mL size).

* Each promo code is valid for a single use. No purchase necessary. Not valid with other promo codes or special offers. Promo codes cannot be used towards the purchase of an OligoCard^® payment card. Valid for direct sales only. Cannot be applied to past purchases. Standard shipping and taxation charges may apply to discounted and free promotional items. Other restrictions may apply. Promo code expires December 31, 2019.