Order Menu

User guides and protocols

DNA & RNA

PDF tag

Reduction of thiol-modified oligonucleotides (575 KB)

PDF tag

Annealing oligonucleotides (503 KB)

PDF tag

Resuspension of duplexed oligonucleotides (267 KB)

CRISPR genome editing

PDF tag

Homology-directed repair using Alt-R CRISPR-Cas9 System and Ultramer Oligos (974 KB)

PDF tag

Alt-R CRISPR-Cas9 system—RNP transfections (recommended) (1236 KB)

PDF tag

Alt-R CRISPR-Cas9 system—RNP electroporation, Amaxa Nucleofector system (503 KB)

PDF tag

Alt-R CRISPR-Cas9 system—RNP electroporation, Neon Transfection system (414 KB)

PDF tag

Alt-R CRISPR-Cas9 system—RNP electroporation, Gene Pulser Xcell system (503 KB)

PDF tag

Alt-R CRISPR-Cas9 system—in vitro cleavage of target DNA with RNP complex (577 KB)

PDF tag

Alt-R CRISPR-Cas9 system—plasmid transfections (430 KB)

PDF tag

Alt-R CRISPR-Cpf1—RNP electroporation, Amaxa Nucleofector system (514 KB)

PDF tag

Alt-R CRISPR-Cpf1—RNP electroporation, Neon Transfection system (496 KB)

PDF tag

Alt-R Genome Editing Detection Kit (910 KB)

User-submitted methods

PDF tag

CRISPR-Cas9 RNP delivery—human stem cells—K Moore (165 KB)

PDF tag

CRISPR-Cas9 RNP delivery—iPS cell electroporation—NepaGene (210 KB)

PDF tag

CRISPR-Cas9 RNP delivery—CD34+ hematopoietic stem and progenitor cells—A Hendel (375 KB)

PDF tag

CRISPR-Cas9 RNP delivery—mouse zygote electroporation—NepaGene (428 KB)

PDF tag

CRISPR-Cas9 RNP delivery—mouse zygote electroporation—Takemoto (176 KB)

PDF tag

CRISPR-Cas9 RNP delivery—mouse zygote microinjection—CB Gurumurthy (505 KB)

PDF tag

CRISPR-Cas9 RNP delivery—zebrafish embryos—J Essner (119 KB)

PDF tag

CRISPR-Cas9 RNP delivery—B. tryoni microinjection—A Choo (179 KB)

PDF tag

CRISPR-Cas9 RNP delivery—C. elegans—A Dernburg (123 KB)

Next generation sequencing

Library prep

IDT Lotus DNA Library Prep Kit Protocol (2497 KB)

Hybridization capture

xGen hybridization capture of DNA libraries for NGS target enrichment (3929 KB)

Automation of xGen hybridization capture on the Sciclone G3 NGS Workstation (2459 KB)

Legacy protocols—hybridization capture

PDF tag

Hybridization capture of Nextera DNA libraries using xGen Lockdown Probes and Panels (490 KB)

PDF tag

xGen Blocking Oligos resuspension protocol (506 KB)

PDF tag

xGen Lockdown Probes and Panels resuspension protocol (664 KB)

PDF tag

Expansion of xGen Lockdown Panels with xGen spike-in panels (681 KB)

PDF tag

Hybridization capture of TruSeq libraries using xGen Lockdown Probes and Reagents (1960 KB)

Data analysis guidelines—hybridization capture

PDF tag

Demultiplexing data containing unique molecular identifiers (UMIs) (1869 KB)

PDF tag

Variant calling data containing unique molecular identifiers (UMIs) (1813 KB)

Amplicon sequencing

PDF tag

High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (1235 KB)

PDF tag

Quick reference guide: High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (965 KB)

PDF tag

rhAmpSeq library preparation for targeted amplicon sequencing (1620 KB)

PDF tag

Quick reference guide: rhAmpSeq library preparation for targeted amplicon sequencing (967 KB)

Data analysis guidelines—amplicon sequencing

PDF tag

Analysis Guideline: Genotype and variant calling with rhAmpSeq sequencing data (1135 KB)

PDF tag

Analysis Guideline: Evaluate CRISPR DNA editing with rhAmpSeq sequencing data (903 KB)

Sample data—amplicon sequencing

Genotyping sample data—rhAmpSeq analysis (gz)

Download (41511 KB)

CRISPR sample data–rhAmpSeq analysis (gz)

Download (36133 KB)

Genes & gene fragments

PDF tag

gBlocks Gene Fragment T/A cloning (141 KB)

PDF tag

Gibson Assembly method (178 KB)

qPCR & PCR

PDF tag

rhAmp SNP Genotyping (670 KB)

PDF tag

rhPCR (436 KB)

PDF tag

Dye calibration—AB 7300 and 7500/7500 Fast (139 KB)

PDF tag

PrimeTime qPCR Assay Plate resuspension (507 KB)

PDF tag

PrimeTime Gene Expression Master Mix (149 KB)

PDF tag

Dye calibration—AB 7900 Fast (179 KB)

PDF tag

PrimeTime Primer Plate resuspension (500 KB)

PDF tag

PrimeTime qPCR Assay resuspension (504 KB)

PDF tag

PrimeTime qPCR Primers resuspension (499 KB)

Functional genomics

PDF tag

IDT miRNA Inhibitors resuspension (496 KB)

PDF tag

TriFECTa RNAi kits (1044 KB)

PDF tag

Resuspension of duplexed oligonucleotides (267 KB)

Reagents & kits

PDF tag

Surveyor Kit for gel electrophoresis (481 KB)

PDF tag

DNaseAlert nuclease detection (1399 KB)

PDF tag

RNaseAlert nuclease detection (609 KB)

PDF tag

Nuclease Decontamination Solution (495 KB)

PrimeTime Gene Expression Master Mix

qPCR gene expression master mix

Use promo code FREEMASTERMIX* at checkout to receive a free vial of PrimeTime Gene Expression Master Mix (1 mL size).

* Each promo code is valid for a single use. No purchase necessary. Not valid with other promo codes or special offers. Promo codes cannot be used towards the purchase of an OligoCard^® payment card. Valid for direct sales only. Cannot be applied to past purchases. Standard shipping and taxation charges may apply to discounted and free promotional items. Other restrictions may apply. Promo code expires December 31, 2019.