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User guides and protocols

DNA & RNA

PDF tag Reduction of thiol-modified oligonucleotides (709 KB)
PDF tag Reduction of thiol-modified oligonucleotides (709 KB)
PDF tag Resuspension of duplexed oligonucleotides (267 KB)
PDF tag Resuspension of duplexed oligonucleotides (267 KB)
PDF tag Annealing oligonucleotides (503 KB)
PDF tag Annealing oligonucleotides (503 KB)
User-submitted methods
Cloning high-quality CRISPR libraries with oPools Oligo Pools—B Herken (329 KB)

qPCR & PCR

PDF tag rhAmp SNP Genotyping (670 KB)
PDF tag rhAmp SNP Genotyping (670 KB)
PDF tag rhPCR (436 KB)
PDF tag rhPCR (436 KB)
PDF tag Dye calibration—AB 7300 and 7500/7500 Fast (139 KB)
PDF tag Dye calibration—AB 7300 and 7500/7500 Fast (139 KB)
PDF tag PrimeTime qPCR Assay Plate resuspension (507 KB)
PDF tag PrimeTime qPCR Assay Plate resuspension (507 KB)
PDF tag PrimeTime Gene Expression Master Mix (149 KB)
PDF tag PrimeTime Gene Expression Master Mix (149 KB)
PDF tag Dye calibration—AB 7900 Fast (179 KB)
PDF tag Dye calibration—AB 7900 Fast (179 KB)
PDF tag PrimeTime Primer Plate resuspension (500 KB)
PDF tag PrimeTime Primer Plate resuspension (500 KB)
PDF tag PrimeTime qPCR Assay resuspension (504 KB)
PDF tag PrimeTime qPCR Assay resuspension (504 KB)
PDF tag PrimeTime qPCR Primers resuspension (493 KB)
PDF tag PrimeTime qPCR Primers resuspension (493 KB)

CRISPR genome editing

Cas9
PDF tag Alt-R CRISPR-Cas9 system—RNP transfections (recommended) (1236 KB)
PDF tag Alt-R CRISPR-Cas9 system—RNP transfections (recommended) (1236 KB)
PDF tag Alt-R CRISPR-Cas9 system—RNP electroporation, Amaxa Nucleofector system (797 KB)
PDF tag Alt-R CRISPR-Cas9 system—RNP electroporation, Amaxa Nucleofector system (797 KB)
PDF tag Alt-R CRISPR-Cas9 system—RNP electroporation, Neon Transfection system (862 KB)
PDF tag Alt-R CRISPR-Cas9 system—RNP electroporation, Neon Transfection system (862 KB)
PDF tag Alt-R CRISPR-Cas9 system—RNP electroporation, Gene Pulser Xcell system (503 KB)
PDF tag Alt-R CRISPR-Cas9 system—RNP electroporation, Gene Pulser Xcell system (503 KB)
PDF tag Alt-R CRISPR-Cas9 system—in vitro cleavage of target DNA with RNP complex (581 KB)
PDF tag Alt-R CRISPR-Cas9 system—in vitro cleavage of target DNA with RNP complex (581 KB)
PDF tag Alt-R CRISPR-Cas9 system—plasmid transfections (430 KB)
PDF tag Alt-R CRISPR-Cas9 system—plasmid transfections (430 KB)
PDF tag Alt-R Genome Editing Detection Kit (910 KB)
PDF tag Alt-R Genome Editing Detection Kit (910 KB)
Homology-directed repair (HDR)
PDF tag Homology-directed repair using the Alt-R CRISPR-Cas9 System and Megamer ssDNA Fragments (1433 KB)
PDF tag Homology-directed repair using the Alt-R CRISPR-Cas9 System and Megamer ssDNA Fragments (1433 KB)
PDF tag Homology-directed repair using the Alt-R CRISPR-Cas9 System and HDR Donor Oligos (1328 KB)
PDF tag Homology-directed repair using the Alt-R CRISPR-Cas9 System and HDR Donor Oligos (1328 KB)
Cas12a (Cpf1)
PDF tag Alt-R CRISPR-Cpf1—RNP electroporation, Amaxa Nucleofector system (514 KB)
PDF tag Alt-R CRISPR-Cpf1—RNP electroporation, Amaxa Nucleofector system (514 KB)
PDF tag Alt-R CRISPR-Cpf1—RNP electroporation, Neon Transfection system (496 KB)
PDF tag Alt-R CRISPR-Cpf1—RNP electroporation, Neon Transfection system (496 KB)
Mutation detection
PDF tag Alt-R Genome Editing Detection Kit (910 KB)
PDF tag Alt-R Genome Editing Detection Kit (910 KB)
User-submitted methods

Next generation sequencing

Library prep
Hybridization capture
Legacy protocols—hybridization capture
PDF tag Hybridization capture of Nextera DNA libraries using xGen Lockdown Probes and Panels (490 KB)
PDF tag Hybridization capture of Nextera DNA libraries using xGen Lockdown Probes and Panels (490 KB)
PDF tag xGen Blocking Oligos resuspension protocol (506 KB)
PDF tag xGen Blocking Oligos resuspension protocol (506 KB)
PDF tag xGen Lockdown Probes and Panels resuspension protocol (664 KB)
PDF tag xGen Lockdown Probes and Panels resuspension protocol (664 KB)
PDF tag Expansion of xGen Lockdown Panels with xGen spike-in panels (681 KB)
PDF tag Expansion of xGen Lockdown Panels with xGen spike-in panels (681 KB)
PDF tag Hybridization capture of TruSeq libraries using xGen Lockdown Probes and Reagents (1960 KB)
PDF tag Hybridization capture of TruSeq libraries using xGen Lockdown Probes and Reagents (1960 KB)
PDF tag Analysis guideline: xGen Prism DNA Library Prep Kit (1728 KB)
PDF tag Analysis guideline: xGen Prism DNA Library Prep Kit (1728 KB)
PDF tag Analysis guideline: Demultiplexing data containing unique molecular identifiers (UMIs) (1869 KB)
PDF tag Analysis guideline: Demultiplexing data containing unique molecular identifiers (UMIs) (1869 KB)
PDF tag Analysis guideline: Variant calling data containing unique molecular identifiers (UMIs) (1813 KB)
PDF tag Analysis guideline: Variant calling data containing unique molecular identifiers (UMIs) (1813 KB)
Amplicon sequencing
PDF tag Quick reference guide: Pooling multiple rhAmpSeq Panels (802 KB)
PDF tag Quick reference guide: Pooling multiple rhAmpSeq Panels (802 KB)
PDF tag High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (1235 KB)
PDF tag High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (1235 KB)
PDF tag Quick reference guide: High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (965 KB)
PDF tag Quick reference guide: High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (965 KB)
PDF tag rhAmpSeq library preparation for targeted amplicon sequencing (1620 KB)
PDF tag rhAmpSeq library preparation for targeted amplicon sequencing (1620 KB)
PDF tag Quick reference guide: rhAmpSeq library preparation for targeted amplicon sequencing (990 KB)
PDF tag Quick reference guide: rhAmpSeq library preparation for targeted amplicon sequencing (990 KB)
Data analysis guidelines—amplicon sequencing
PDF tag Analysis guideline: Genotype and variant calling with rhAmpSeq sequencing data (1135 KB)
PDF tag Analysis guideline: Genotype and variant calling with rhAmpSeq sequencing data (1135 KB)
PDF tag Analysis guideline: Evaluate CRISPR DNA editing with rhAmpSeq sequencing data (903 KB)
PDF tag Analysis guideline: Evaluate CRISPR DNA editing with rhAmpSeq sequencing data (903 KB)
Sample data—amplicon sequencing
Genotyping sample data—rhAmpSeq analysis (gz)
Download (41511 KB)
CRISPR sample data–rhAmpSeq analysis (gz)
Download (36133 KB)

Genes & gene fragments

PDF tag Blunt-end cloning method protocol (853 KB)
PDF tag Blunt-end cloning method protocol (853 KB)
PDF tag TA cloning method protocol (828 KB)
PDF tag TA cloning method protocol (828 KB)
PDF tag Gibson Assembly method (908 KB)
PDF tag Gibson Assembly method (908 KB)
User-submitted methods
PDF tag Cloning high-quality CRISPR libraries with oPools Oligo Pools—B Herken (329 KB)
PDF tag Cloning high-quality CRISPR libraries with oPools Oligo Pools—B Herken (329 KB)

Functional genomics

PDF tag IDT miRNA Inhibitors resuspension (496 KB)
PDF tag IDT miRNA Inhibitors resuspension (496 KB)
PDF tag TriFECTa RNAi kits (1044 KB)
PDF tag TriFECTa RNAi kits (1044 KB)
PDF tag Resuspension of duplexed oligonucleotides (267 KB)
PDF tag Resuspension of duplexed oligonucleotides (267 KB)

Reagents & kits

PDF tag Surveyor Kit for gel electrophoresis (481 KB)
PDF tag Surveyor Kit for gel electrophoresis (481 KB)
PDF tag DNaseAlert nuclease detection (483 KB)
PDF tag DNaseAlert nuclease detection (483 KB)
PDF tag RNaseAlert nuclease detection (445 KB)
PDF tag RNaseAlert nuclease detection (445 KB)
PDF tag Nuclease Decontamination Solution (495 KB)
PDF tag Nuclease Decontamination Solution (495 KB)

PrimeTime Gene Expression Master Mix

qPCR gene expression master mix

Use promo code FREEMASTERMIX* at checkout to receive a free vial of PrimeTime Gene Expression Master Mix (1 mL size).

* Each promo code is valid for a single use. No purchase necessary. Not valid with other promo codes or special offers. Promo codes cannot be used towards the purchase of an OligoCard® payment card. Valid for direct sales only. Cannot be applied to past purchases. Standard shipping and taxation charges may apply to discounted and free promotional items. Other restrictions may apply. Promo code expires December 31, 2020.