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User guides and protocols

DNA & RNA

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Reduction of thiol-modified oligonucleotides (575 KB)

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Annealing oligonucleotides (503 KB)

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Resuspension of duplexed oligonucleotides (267 KB)

CRISPR genome editing

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Homology-directed repair using Alt-R CRISPR-Cas9 System and Ultramer Oligos (974 KB)

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Alt-R CRISPR-Cas9 system—RNP transfections (recommended) (1236 KB)

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Alt-R CRISPR-Cas9 system—RNP electroporation, Amaxa Nucleofector system (503 KB)

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Alt-R CRISPR-Cas9 system—RNP electroporation, Neon Transfection system (414 KB)

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Alt-R CRISPR-Cas9 system—RNP electroporation, Gene Pulser Xcell system (503 KB)

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Alt-R CRISPR-Cas9 system—in vitro cleavage of target DNA with RNP complex (420 KB)

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Alt-R CRISPR-Cas9 system—plasmid transfections (430 KB)

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Alt-R CRISPR-Cpf1—RNP electroporation, Amaxa Nucleofector system (514 KB)

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Alt-R CRISPR-Cpf1—RNP electroporation, Neon Transfection system (496 KB)

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Alt-R Genome Editing Detection Kit (910 KB)

User-submitted methods

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CRISPR-Cas9 RNP delivery—human stem cells—K Moore (165 KB)

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CRISPR-Cas9 RNP delivery—iPS cell electroporation—NepaGene (210 KB)

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CRISPR-Cas9 RNP delivery—CD34+ hematopoietic stem and progenitor cells—A Hendel (375 KB)

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CRISPR-Cas9 RNP delivery—mouse zygote electroporation—NepaGene (158 KB)

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CRISPR-Cas9 RNP delivery—mouse zygote electroporation—Takemoto (176 KB)

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CRISPR-Cas9 RNP delivery—mouse zygote microinjection—CB Gurumurthy (505 KB)

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CRISPR-Cas9 RNP delivery—zebrafish embryos—J Essner (119 KB)

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CRISPR-Cas9 RNP delivery—B. tryoni microinjection—A Choo (179 KB)

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CRISPR-Cas9 RNP delivery—C. elegans—A Dernburg (123 KB)

Next generation sequencing

xGen hybridization capture of DNA libraries for NGS target enrichment (3955 KB)

Automation of xGen hybridization capture on the Sciclone G3 NGS Workstation (4302 KB)

Legacy protocols

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Alt-R CRISPR-Cpf1—RNP electroporation, Amaxa Nucleofector system (514 KB)

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Alt-R CRISPR-Cpf1—RNP electroporation, Neon Transfection system (496 KB)

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Alt-R Genome Editing Detection Kit (910 KB)

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Hybridization capture of Nextera DNA libraries using xGen Lockdown Probes and Panels (490 KB)

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xGen Blocking Oligos resuspension protocol (506 KB)

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xGen Lockdown Probes and Panels resuspension protocol (664 KB)

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Expansion of xGen Lockdown Panels with xGen spike-in panels (681 KB)

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Hybridization capture of TruSeq libraries using xGen Lockdown Probes and Reagents (1960 KB)

Data analysis guidelines

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Demultiplexing data containing unique molecular identifiers (UMIs) (1869 KB)

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Variant calling data containing unique molecular identifiers (UMIs) (1813 KB)

Genes & gene fragments

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gBlocks Gene Fragment T/A cloning (141 KB)

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Gibson Assembly method (178 KB)

qPCR & PCR

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rhAmp SNP Genotyping (670 KB)

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rhPCR (436 KB)

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Dye calibration—AB 7300 and 7500/7500 Fast (139 KB)

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PrimeTime qPCR Assay Plate resuspension (507 KB)

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PrimeTime Gene Expression Master Mix (149 KB)

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Dye calibration—AB 7900 Fast (179 KB)

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PrimeTime Primer Plate resuspension (500 KB)

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PrimeTime qPCR Assay resuspension (504 KB)

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PrimeTime qPCR Primers resuspension (499 KB)

Functional genomics

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IDT miRNA Inhibitors resuspension (496 KB)

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TriFECTa RNAi kits (1044 KB)

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Resuspension of duplexed oligonucleotides (267 KB)

Reagents & kits

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Surveyor Kit for gel electrophoresis (481 KB)

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DNaseAlert nuclease detection (1399 KB)

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RNaseAlert nuclease detection (609 KB)

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Nuclease Decontamination Solution (495 KB)

PrimeTime Gene Expression Master Mix

PrimeTime-Gene-Expression-Master-Mix

Use promo code FREEMASTERMIX* at checkout to receive a free vial of PrimeTime Gene Expression Master Mix (1 mL size).

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