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User guides and protocols

DNA & RNA

Reduction of thiol-modified oligonucleotides (709 KB)

Reduction of thiol-modified oligonucleotides (709 KB)

Resuspension of duplexed oligonucleotides (267 KB)

Resuspension of duplexed oligonucleotides (267 KB)

Annealing oligonucleotides (194 KB)

Annealing oligonucleotides (194 KB)

User-submitted methods

Cloning high-quality CRISPR libraries with oPools Oligo Pools Demonstrated Protocol (396 KB)

qPCR & PCR

PrimeTime One-Step 4X Broad-Range Master Mix Protocol (522 KB)

PrimeTime One-Step 4X Broad-Range Master Mix Protocol (522 KB)

rhAmp SNP Genotyping (670 KB)

rhAmp SNP Genotyping (670 KB)

rhPCR (436 KB)

rhPCR (436 KB)

qPCR Dye calibration on AB systems protocol (609 KB)

qPCR Dye calibration on AB systems protocol (609 KB)

PrimeTime qPCR Assay Plate resuspension (507 KB)

PrimeTime qPCR Assay Plate resuspension (507 KB)

PrimeTime Gene Expression Master Mix (149 KB)

PrimeTime Gene Expression Master Mix (149 KB)

PrimeTime Primer Plate resuspension (500 KB)

PrimeTime Primer Plate resuspension (500 KB)

PrimeTime qPCR Assay resuspension (504 KB)

PrimeTime qPCR Assay resuspension (504 KB)

PrimeTime qPCR Primers resuspension (493 KB)

PrimeTime qPCR Primers resuspension (493 KB)

CRISPR genome editing

Cas9

Homology-directed repair using the Alt-R CRISPR-Cas9 System and Alt-R HDR Donor Blocks (1.3 MB)

Homology-directed repair using the Alt-R CRISPR-Cas9 System and Alt-R HDR Donor Blocks (1.3 MB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 System and HDR Donor Oligos (878 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 System and HDR Donor Oligos (878 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Neon] (839 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Neon] (839 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Nucleofector] (879 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Nucleofector] (879 KB)

Alt-R CRISPR-Cas9 System: Cationic lipid delivery of CRISPR ribonucleoprotein complexes into mammalian cells (1.2 MB)

Alt-R CRISPR-Cas9 System: Cationic lipid delivery of CRISPR ribonucleoprotein complexes into mammalian cells (1.2 MB)

Alt-R CRISPR-Cas9_Delivery of ribonucleoprotein complexes into HEK-293 cells using the Lonza Nucleofector System (968 KB)

Alt-R CRISPR-Cas9_Delivery of ribonucleoprotein complexes into HEK-293 cells using the Lonza Nucleofector System (968 KB)

Alt-R CRISPR-Cas9 system—RNP electroporation, Neon Transfection system (508 KB)

Alt-R CRISPR-Cas9 system—RNP electroporation, Neon Transfection system (508 KB)

Alt-R CRISPR-Cas9 system—RNP electroporation, Gene Pulser Xcell system (585 KB)

Alt-R CRISPR-Cas9 system—RNP electroporation, Gene Pulser Xcell system (585 KB)

Alt-R CRISPR-Cas9 system—in vitro cleavage of target DNA with RNP complex (408 KB)

Alt-R CRISPR-Cas9 system—in vitro cleavage of target DNA with RNP complex (408 KB)

Alt-R Genome Editing Detection Kit (1.3 MB)

Alt-R Genome Editing Detection Kit (1.3 MB)

Homology-directed repair (HDR)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 System and HDR Donor Oligos (878 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 System and HDR Donor Oligos (878 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Neon] (839 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Neon] (839 KB)

Homology-directed repair using the Alt-R CRISPR-Cas9 System and Megamer ssDNA Fragments (1.3 MB)

Homology-directed repair using the Alt-R CRISPR-Cas9 System and Megamer ssDNA Fragments (1.3 MB)

Homology-directed repair using the Alt-R CRISPR-Cas9 System and HDR Donor Oligos (1.1 MB)

Homology-directed repair using the Alt-R CRISPR-Cas9 System and HDR Donor Oligos (1.1 MB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Nucleofector] (879 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Neon] (839 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 System and HDR Donor Oligos (878 KB)

Legacy protocols/guides for Alt-R HDR Enhancer

Cas12a (Cpf1)

Alt-R CRISPR-Cas12a Cpf1 System_Delivery of ribonucleoprotein complexes into Jurkat T cells using the Neon Transfection System Protocol (730 KB)

Alt-R CRISPR-Cas12a Cpf1 System_Delivery of ribonucleoprotein complexes into Jurkat T cells using the Neon Transfection System Protocol (730 KB)

Alt-R CRISPR-Cpf1—RNP electroporation, Amaxa Nucleofector system (514 KB)

Alt-R CRISPR-Cpf1—RNP electroporation, Amaxa Nucleofector system (514 KB)

Mutation detection

Alt-R Genome Editing Detection Kit (1.3 MB)

Alt-R Genome Editing Detection Kit (1.3 MB)

Next generation sequencing

Library prep

xGen UDI Primer plates Quick Reference Guide (610 KB)

xGen UDI Primer plates Quick Reference Guide (610 KB)

Input DNA Quantification Assay Demonstrated Protocol (412 KB)

Input DNA Quantification Assay Demonstrated Protocol (412 KB)

xGen cfDNA and FFPE DNA Library Prep Kit Analysis Guidelines (1.6 MB)

xGen cfDNA and FFPE DNA Library Prep Kit Analysis Guidelines (1.6 MB)

Quick reference guide: xGen Stubby Adapter and Indexing Primers (610 KB)

Quick reference guide: xGen Stubby Adapter and Indexing Primers (610 KB)

xGen Adaptase Module Protocol (1 MB)

xGen Adaptase Module Protocol (1 MB)

xGen Broad-Range RNA Library Prep Kit Protocol (1.7 MB)

xGen Broad-Range RNA Library Prep Kit Protocol (1.7 MB)

xGen DNA Library Prep EZ Kit and xGen DNA Library Prep EZ UNI Kit Protocol (1.7 MB)

xGen DNA Library Prep EZ Kit and xGen DNA Library Prep EZ UNI Kit Protocol (1.7 MB)

xGen DNA Library Prep MC Kit and xGen DNA Library Prep MC UNI Kit Protocol (1.2 MB)

xGen DNA Library Prep MC Kit and xGen DNA Library Prep MC UNI Kit Protocol (1.2 MB)

xGen cfDNA and FFPE DNA Library Prep v2 MC Kit Protocol (2.1 MB)

xGen cfDNA and FFPE DNA Library Prep v2 MC Kit Protocol (2.1 MB)

xGen Normalase Module Protocol (1.6 MB)

xGen Normalase Module Protocol (1.6 MB)

xGen ssDNA and Low-Input DNA Library Prep Kit Protocol (1.1 MB)

xGen ssDNA and Low-Input DNA Library Prep Kit Protocol (1.1 MB)

xGen Methyl-Seq DNA Library Prep Kit Protocol (1021 KB)

xGen Methyl-Seq DNA Library Prep Kit Protocol (1021 KB)

xGen Deceleration Module Protocol (722 KB)

xGen Deceleration Module Protocol (722 KB)

Quick reference guide: xGen Stubby Adapter and UDI Primer Pairs (670 KB)

Quick reference guide: xGen Stubby Adapter and UDI Primer Pairs (670 KB)

xGen UDI-UMI Adapters quick reference guide (778 KB)

xGen UDI-UMI Adapters quick reference guide (778 KB)

Analysis guideline: Demultiplexing Illumina sequencing data with UMIs (893 KB)

Analysis guideline: Demultiplexing Illumina sequencing data with UMIs (893 KB)

Analysis guideline: xGen UDI-UMI Adapters-Processing sequence data with unique molecular identifiers (UMIs).pdf (798 KB)

Analysis guideline: xGen UDI-UMI Adapters-Processing sequence data with unique molecular identifiers (UMIs).pdf (798 KB)

Analysis guidelines—library prep

Analysis guideline: xGen Prism DNA Library Prep Kit (1.7 MB)

Analysis guideline: xGen UDI-UMI Adapters-Processing sequence data with unique molecular identifiers (UMIs).pdf (798 KB)

Analysis guideline: Demultiplexing Illumina sequencing data with UMIs (893 KB)

Legacy protocols—library prep

Quick reference protocol: xGen Prism DNA Library Prep Kit (1.2 MB)

Quick reference guide: xGen Stubby Adapter and UDI Primer Pairs (670 KB)

Hybridization capture

xGen Hyb Panel Design Tool User Guide (1.1 MB)

xGen Hyb Panel Design Tool User Guide (1.1 MB)

xGen hybridization capture of Illumina Nextera DNA libraries Protocol (2.9 MB)

xGen hybridization capture of Illumina Nextera DNA libraries Protocol (2.9 MB)

xGen Hybridization Capture of DNA Libraries Protocol (2.9 MB)

xGen Hybridization Capture of DNA Libraries Protocol (2.9 MB)

Automation of xGen Hybridization Capture on the Sciclone G3 NGS Workstation Protocol (1.4 MB)

Automation of xGen Hybridization Capture on the Sciclone G3 NGS Workstation Protocol (1.4 MB)

Analysis Guideline: Processing sequence data for copy number variations (CNVs) (1.4 MB)

Analysis Guideline: Processing sequence data for copy number variations (CNVs) (1.4 MB)

Legacy protocols—hybridization capture

Expansion of xGen Lockdown Panels with xGen spike-in panels (681 KB)

Amplicon sequencing

xGen Amplicon Panels for viral genome sequencing Protocol (1.5 MB)

xGen Amplicon Panels for viral genome sequencing Protocol (1.5 MB)

Sequencing SARS-CoV-2 with the Midnight Amplicon Panel Demonstrated Protocol (1.1 MB)

Sequencing SARS-CoV-2 with the Midnight Amplicon Panel Demonstrated Protocol (1.1 MB)

xGen DNA Library Prep: Sequencing SARS-CoV-2 with the ARTIC nCoV-2019 Amplicon Panel Demonstrated Protocol (1 MB)

xGen DNA Library Prep: Sequencing SARS-CoV-2 with the ARTIC nCoV-2019 Amplicon Panel Demonstrated Protocol (1 MB)

xGen 16S Amplicon Panel v2 and xGen ITS1 Amplicon Panel Protocol (1.2 MB)

xGen 16S Amplicon Panel v2 and xGen ITS1 Amplicon Panel Protocol (1.2 MB)

xGen Amplicon Panels for Oncology Research, Inherited Disease Research, and Sample Tracking Protocol (1.1 MB)

xGen Amplicon Panels for Oncology Research, Inherited Disease Research, and Sample Tracking Protocol (1.1 MB)

xGen SARS-CoV-2 Amplicon Panel and xGen SARS-CoV-2 S Gene Amplicon Panel Protocol (1.3 MB)

xGen SARS-CoV-2 Amplicon Panel and xGen SARS-CoV-2 S Gene Amplicon Panel Protocol (1.3 MB)

xGen HS EGFR Pathway Amplicon Panel Protocol (1.1 MB)

xGen HS EGFR Pathway Amplicon Panel Protocol (1.1 MB)

Legacy protocols—amplicon sequencing

Legacy data analysis guidelines—amplicon sequencing

Legacy sample data—amplicon sequencing

Genotyping sample data—rhAmpSeq analysis (40.5 MB)

Genes & gene fragments

Blunt-end cloning method protocol (853 KB)

Blunt-end cloning method protocol (853 KB)

TA cloning method protocol (828 KB)

TA cloning method protocol (828 KB)

User-submitted methods

Cloning high-quality CRISPR libraries with oPools Oligo Pools Demonstrated Protocol (396 KB)

Cloning high-quality CRISPR libraries with oPools Oligo Pools Demonstrated Protocol (396 KB)

Functional genomics

IDT miRNA Inhibitors resuspension (496 KB)

IDT miRNA Inhibitors resuspension (496 KB)

TriFECTa RNAi kits (1 MB)

TriFECTa RNAi kits (1 MB)

Resuspension of duplexed oligonucleotides (267 KB)

Resuspension of duplexed oligonucleotides (267 KB)

Reagents & kits

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.

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