SARS-CoV-2. Don’t let up. We’ll help.

User guides and protocols

DNA & RNA

Reduction of thiol-modified oligonucleotides (709 KB)

Reduction of thiol-modified oligonucleotides (709 KB)

Resuspension of duplexed oligonucleotides (267 KB)

Resuspension of duplexed oligonucleotides (267 KB)

Annealing oligonucleotides (503 KB)

Annealing oligonucleotides (503 KB)

User-submitted methods

Cloning high-quality CRISPR libraries with oPools Oligo Pools—B Herken (329 KB)

qPCR & PCR

rhAmp SNP Genotyping (670 KB)

rhAmp SNP Genotyping (670 KB)

rhPCR (436 KB)

rhPCR (436 KB)

Dye calibration—AB 7300 and 7500/7500 Fast (139 KB)

Dye calibration—AB 7300 and 7500/7500 Fast (139 KB)

PrimeTime qPCR Assay Plate resuspension (507 KB)

PrimeTime qPCR Assay Plate resuspension (507 KB)

PrimeTime Gene Expression Master Mix (149 KB)

PrimeTime Gene Expression Master Mix (149 KB)

Dye calibration—AB 7900 Fast (179 KB)

Dye calibration—AB 7900 Fast (179 KB)

PrimeTime Primer Plate resuspension (500 KB)

PrimeTime Primer Plate resuspension (500 KB)

PrimeTime qPCR Assay resuspension (504 KB)

PrimeTime qPCR Assay resuspension (504 KB)

PrimeTime qPCR Primers resuspension (493 KB)

PrimeTime qPCR Primers resuspension (493 KB)

CRISPR genome editing

Cas9

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 System and HDR Donor Oligos (881 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 System and HDR Donor Oligos (881 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Neon] (841 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Neon] (841 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Nucleofector] (878 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Nucleofector] (878 KB)

Alt-R CRISPR-Cas9 system—RNP transfections (recommended) (1.2 MB)

Alt-R CRISPR-Cas9 system—RNP transfections (recommended) (1.2 MB)

Alt-R CRISPR-Cas9_Delivery of ribonucleoprotein complexes into HEK-293 cells using the Lonza Nucleofector System (969 KB)

Alt-R CRISPR-Cas9_Delivery of ribonucleoprotein complexes into HEK-293 cells using the Lonza Nucleofector System (969 KB)

Alt-R CRISPR-Cas9 system—RNP electroporation, Neon Transfection system (862 KB)

Alt-R CRISPR-Cas9 system—RNP electroporation, Neon Transfection system (862 KB)

Alt-R CRISPR-Cas9 system—RNP electroporation, Gene Pulser Xcell system (503 KB)

Alt-R CRISPR-Cas9 system—RNP electroporation, Gene Pulser Xcell system (503 KB)

Alt-R CRISPR-Cas9 system—in vitro cleavage of target DNA with RNP complex (647 KB)

Alt-R CRISPR-Cas9 system—in vitro cleavage of target DNA with RNP complex (647 KB)

Alt-R Genome Editing Detection Kit (1.3 MB)

Alt-R Genome Editing Detection Kit (1.3 MB)

Homology-directed repair (HDR)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 System and HDR Donor Oligos (881 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 System and HDR Donor Oligos (881 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Neon] (841 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Neon] (841 KB)

Homology-directed repair using the Alt-R CRISPR-Cas9 System and Megamer ssDNA Fragments (1.3 MB)

Homology-directed repair using the Alt-R CRISPR-Cas9 System and Megamer ssDNA Fragments (1.3 MB)

Homology-directed repair using the Alt-R CRISPR-Cas9 System and HDR Donor Oligos (1.1 MB)

Homology-directed repair using the Alt-R CRISPR-Cas9 System and HDR Donor Oligos (1.1 MB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Nucleofector] (878 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 and Megamer ssDNA Fragments [Neon] (841 KB)

Quick reference protocol: HDR with Alt-R CRISPR-Cas9 System and HDR Donor Oligos (881 KB)

Legacy protocols/guides for Alt-R HDR Enhancer

Legacy Perform Gene Knockout With the Alt-R CRISPR Cas System Reference Guide (1 MB)

Legacy Perform Gene Knockout With the Alt-R CRISPR Cas System Reference Guide (1 MB)

Legacy Alt-R CRISPR Cpf1 User Guide RNP Electroporation Neon System (496 KB)

Legacy Alt-R CRISPR Cpf1 User Guide RNP Electroporation Neon System (496 KB)

Legacy Alt-R CRISPR Cas9 User Guide Ribonucleoprotein Electroporation Amaxa Nucleofector System (797 KB)

Legacy Alt-R CRISPR Cas9 User Guide Ribonucleoprotein Electroporation Amaxa Nucleofector System (797 KB)

Cas12a (Cpf1)

Alt-R CRISPR-Cpf1—RNP electroporation, Amaxa Nucleofector system (514 KB)

Alt-R CRISPR-Cpf1—RNP electroporation, Amaxa Nucleofector system (514 KB)

Alt-R CRISPR-Cas12a Cpf1 System_Delivery of ribonucleoprotein complexes into Jurkat T cells using the Neon Transfection System Protocol (730 KB)

Alt-R CRISPR-Cas12a Cpf1 System_Delivery of ribonucleoprotein complexes into Jurkat T cells using the Neon Transfection System Protocol (730 KB)

Mutation detection

Alt-R Genome Editing Detection Kit (1.3 MB)

Alt-R Genome Editing Detection Kit (1.3 MB)

Next generation sequencing

Library prep

IDT_Demonstrated Protocol for Sequencing SARS-CoV2 with the ARTIC Panel and Lotus Library Prep Kit (2.2 MB)

IDT Demonstrated protocol for sequencing SARS-CoV-2 with the Midnight Panel and Lotus DNA Library Prep Kit (2.1 MB)

Lotus DNA Library Prep Kit Protocol (2.4 MB)

Quick reference protocol: Lotus DNA Library Prep Kit – Targeted sequencing (1015 KB)

Quick reference protocol: Lotus DNA Library Prep Kit – PCR-amplified (1.1 MB)

Quick reference protocol: Lotus DNA Library Prep Kit – PCR-free (914 KB)

xGen Prism DNA Library Prep Kit protocol (2 MB)

Quick reference protocol: xGen Prism DNA Library Prep Kit (1.2 MB)

Quick reference guide: xGen Stubby Adapter and UDI Primer Pairs (670 KB)

Hybridization capture

xGen hybridization capture of DNA libraries for NGS target enrichment (3.9 MB)

Automation of xGen hybridization capture on the Sciclone G3 NGS Workstation (2.4 MB)

Legacy protocols—hybridization capture

Hybridization capture of Nextera DNA libraries using xGen Lockdown Probes and Panels (490 KB)

Hybridization capture of Nextera DNA libraries using xGen Lockdown Probes and Panels (490 KB)

xGen Blocking Oligos resuspension protocol (506 KB)

xGen Blocking Oligos resuspension protocol (506 KB)

xGen Lockdown Probes and Panels resuspension protocol (664 KB)

xGen Lockdown Probes and Panels resuspension protocol (664 KB)

Expansion of xGen Lockdown Panels with xGen spike-in panels (681 KB)

Expansion of xGen Lockdown Panels with xGen spike-in panels (681 KB)

Hybridization capture of TruSeq libraries using xGen Lockdown Probes and Reagents (1.9 MB)

Hybridization capture of TruSeq libraries using xGen Lockdown Probes and Reagents (1.9 MB)

Analysis guideline: xGen Prism DNA Library Prep Kit (1.7 MB)

Analysis guideline: xGen Prism DNA Library Prep Kit (1.7 MB)

Analysis guideline: Demultiplexing data containing unique molecular identifiers (UMIs) (1.8 MB)

Analysis guideline: Demultiplexing data containing unique molecular identifiers (UMIs) (1.8 MB)

Analysis guideline: Variant calling data containing unique molecular identifiers (UMIs) (1.8 MB)

Analysis guideline: Variant calling data containing unique molecular identifiers (UMIs) (1.8 MB)

Amplicon sequencing

Quick reference guide: Pooling multiple rhAmpSeq Panels (802 KB)

Quick reference guide: Pooling multiple rhAmpSeq Panels (802 KB)

High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (1.2 MB)

High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (1.2 MB)

Quick reference guide: High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (965 KB)

Quick reference guide: High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (965 KB)

rhAmpSeq library preparation for targeted amplicon sequencing (1.6 MB)

rhAmpSeq library preparation for targeted amplicon sequencing (1.6 MB)

Quick reference guide: rhAmpSeq library preparation for targeted amplicon sequencing (990 KB)

Quick reference guide: rhAmpSeq library preparation for targeted amplicon sequencing (990 KB)

Data analysis guidelines—amplicon sequencing

Analysis guideline: Genotype and variant calling with rhAmpSeq sequencing data (1.1 MB)

Analysis guideline: Genotype and variant calling with rhAmpSeq sequencing data (1.1 MB)

Sample data—amplicon sequencing

Genotyping sample data—rhAmpSeq analysis (40.5 MB)

Genes & gene fragments

Blunt-end cloning method protocol (853 KB)

Blunt-end cloning method protocol (853 KB)

TA cloning method protocol (828 KB)

TA cloning method protocol (828 KB)

User-submitted methods

Cloning high-quality CRISPR libraries with oPools Oligo Pools—B Herken (329 KB)

Cloning high-quality CRISPR libraries with oPools Oligo Pools—B Herken (329 KB)

Functional genomics

IDT miRNA Inhibitors resuspension (496 KB)

IDT miRNA Inhibitors resuspension (496 KB)

TriFECTa RNAi kits (1 MB)

TriFECTa RNAi kits (1 MB)

Resuspension of duplexed oligonucleotides (267 KB)

Resuspension of duplexed oligonucleotides (267 KB)

Reagents & kits

Surveyor Kit for gel electrophoresis (481 KB)

Surveyor Kit for gel electrophoresis (481 KB)

DNaseAlert nuclease detection (483 KB)

DNaseAlert nuclease detection (483 KB)

RNaseAlert nuclease detection (589 KB)

RNaseAlert nuclease detection (589 KB)

Nuclease Decontamination Solution (495 KB)

Nuclease Decontamination Solution (495 KB)