Next generation sequencing

Hybridization capture
Legacy protocols—hybridization capture
PDF tag Hybridization capture of Nextera DNA libraries using xGen Lockdown Probes and Panels (490 KB)
PDF tag xGen Blocking Oligos resuspension protocol (506 KB)
PDF tag xGen Lockdown Probes and Panels resuspension protocol (664 KB)
PDF tag Expansion of xGen Lockdown Panels with xGen spike-in panels (681 KB)
PDF tag Hybridization capture of TruSeq libraries using xGen Lockdown Probes and Reagents (1960 KB)
Data analysis guidelines—hybridization capture
PDF tag Demultiplexing data containing unique molecular identifiers (UMIs) (1869 KB)
PDF tag Variant calling data containing unique molecular identifiers (UMIs) (1813 KB)
Amplicon sequencing
PDF tag High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (1235 KB)
PDF tag Quick reference guide: High-throughput rhAmpSeq library preparation for targeted amplicon sequencing (965 KB)
PDF tag rhAmpSeq library preparation for targeted amplicon sequencing (1620 KB)
PDF tag Quick reference guide: rhAmpSeq library preparation for targeted amplicon sequencing (967 KB)
Data analysis guidelines—amplicon sequencing
PDF tag Analysis Guideline: Genotype and variant calling with rhAmpSeq sequencing data (1135 KB)
PDF tag Analysis Guideline: Evaluate CRISPR DNA editing with rhAmpSeq sequencing data (903 KB)
Sample data—amplicon sequencing
Genotyping sample data—rhAmpSeq analysis (gz)
Download (41511 KB)
CRISPR sample data–rhAmpSeq analysis (gz)
Download (36133 KB)

Genes & gene fragments

PDF tag gBlocks Gene Fragment T/A cloning (141 KB)
PDF tag Gibson Assembly method (178 KB)